Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

doi:10.7506/spkx1002-6630-201105037

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (5 ): 172-175.doi: 10.7506/spkx1002-6630-201105037

• Bioengineering • Previous Articles Next Articles

Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

ZHANG Qiu-sheng1，XU Bing-lian2，LIU Lin-de1，CHENG Xian-hao1，GAO Xing-xi1，SHI Ya-li

1. School of Life Science, Ludong University, Yantai 264025, China；2. School of Mathmatics and Information, Ludong
University, Yantai 264025, China；3. Sports Institute, Ludong University, Yantai 264025, China

Received:2010-05-18 Revised:2011-01-21 Online:2011-03-15 Published:2011-03-03
Contact: ZHANG Qiu-sheng E-mail:qiushengzhang@126.com

Abstract

Abstract: Pleurotus cituinopileatus is an important kind of edible medicinal fungi. Totally 18 kinds of Pleurotus cituinopileatus strains were collected from some markets in China. Inter-simple sequence repeat (ISSR) method was used to analyze genetic polymorphism of Pleurotus cituinopileatus strains. A total of 102 DNA fragments were amplified by using eleven ISSR primers, and 78 of these fragments were polymorphic with polymorphism rate of 76.4%. Meanwhile, high-level genetic polymorphism was observed in Pleurotus cituinopileatus and the genetic similarity coefficients (GSC) were between 0.50 and 0.96. These 18 strains were divided into 2 groups at GSC of 0.62 and 7 groups at GSC of 0.75 according to the cluster analysis of UPGMA method. Strain 16 had an independent genetic system. These results will provide a scientific fundament to accelerate genetic breeding and identification of cultivated strains of Pleurotus cituinopileatus.

Key words: Pleurotus cituinopileatus, inter-simple sequence repeat (ISSR), polymorphism, intra-specific relationship

CLC Number:

S646

ZHANG Qiu-sheng1，XU Bing-lian2，LIU Lin-de1，CHENG Xian-hao1，GAO Xing-xi1，SHI Ya-li. Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR[J]. FOOD SCIENCE, 2011, 32(5 ): 172-175.

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Genetic Polymorphism Analysis of Pleurotus cituinopileatus by ISSR

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